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产品概况
| 货号 | EK1156 |
|---|---|
| 产品名称 | Rat CDH2/N-Cadherin ELISA Kit |
| 产品描述 | For quantitative detection of rat Cadherin-2 in , cell lysates |
| 免疫原 | NSO, D160-A724 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <20pg/ml |
| 检测范围 | 0.78ng/ml-50ng/ml |
| 基因名称 | CDH2 |
| 基因全名 | cadherin 2, type 1, N-cadherin (neuronal) |
| Uniprot ID | Q9Z1Y3 |
| 指标别名 | CDw325|CDHN|Neural cadherin|N-cadherin|CD325 |
| 背景资料 | Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1156-CAP | 96-well plate precoated with anti- rat Cadherin-2 antibody | 1 |
| EK1156-ST | Lyophilized recombinant rat Cadherin-2 standard | 50ng/tube×2 |
| EK1156-DA | Biotinylated anti- rat Cadherin-2 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1071 | 7288 | 22148 | 1115 | 7627 | 21622 |
| Standard deviation | 57.83 | 291.52 | 885.92 | 63.55 | 312.7 | 1145.96 |
| CV(%) | 5.4% | 4% | 4% | 5.7% | 4.1% | 5.3% |
Rat CDH2/N-Cadherin ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)
| Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.179 | 0.230 | 0.259 | 0.323 | 0.474 | 0.817 | 1.264 | 1.825 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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