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产品概况
| 货号 | EK0926 |
|---|---|
| 产品名称 | Human CD244/2B4 ELISA Kit |
| 产品描述 | For quantitative detection of human CD244 in , cell lysates |
| 免疫原 | NSO, C22-R221 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | CD244 |
| 基因全名 | CD244 molecule, natural killer cell receptor 2B4 |
| Uniprot ID | Q9BZW8 |
| 指标别名 | 2B4|C9.1|CD244|CD244 antigen|CD244 molecule natural killer cell receptor 2B4|CD244 natural killer cell receptor 2B4|CD244_HUMAN|F730046O15Rik|h2B4|Ly90|Natural killer cell activation-inducing ligand|Natural killer cell receptor 2B4|NK cell activation inducing ligand|NK cell activation inducing ligand NAIL|NK cell activation-inducing ligand|NK cell type I receptor protein 2B4|NKR2B4|Nmrk|Non-MHC restricted killing associated|OTTHUMP00000027884|p38|signaling lymphocytic activation molecule 4|SLAM family, member 4|SLAMF4 |
| 背景资料 | CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0926-CAP | 96-well plate precoated with anti- human CD244 antibody | 1 |
| EK0926-ST | Lyophilized recombinant human CD244 standard | 10ng/tube×2 |
| EK0926-DA | Biotinylated anti- human CD244 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 28 | 81 | 453 | 30 | 112 | 469 |
| Standard deviation | 1.34 | 4.62 | 20.84 | 1.53 | 6.61 | 30.02 |
| CV(%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Human CD244/2B4 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.199 | 0.292 | 0.464 | 0.934 | 1.510 | 2.026 | 2.547 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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