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产品概况
| 货号 | EK1188 |
|---|---|
| 产品名称 | Mouse ACE2 ELISA Kit |
| 产品描述 | For quantitative detection of Mouse ACE2 in , serum and plasma(heparin). |
| 免疫原 | CHO, Q18-T740 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 62.5pg/ml-4000pg/ml |
| 基因名称 | ACE2 |
| 基因全名 | angiotensin I converting enzyme 2 |
| Uniprot ID | Q8R0I0 |
| 指标别名 | ACE 2|ACE related carboxypeptidase|ACE-related carboxypeptidase|ACE2|ACE2_HUMAN|ACEH|Angiotensin converting enzyme 2|Angiotensin converting enzyme homolog|Angiotensin converting enzyme like protein|Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2|Angiotensin I converting enzyme 2|Angiotensin-converting enzyme homolog|DKFZP434A014|EC 3.4.17|metalloprotease MPROT 15|Metalloprotease MPROT15|OTTHUMP00000022963|Processed angiotensin-converting enzyme 2 |
| 背景资料 | AngiotensinI-converting enzyme 2(ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1188-CAP | 96-well plate precoated with anti- mouse ACE2 antibody | 1 |
| EK1188-ST | Lyophilized recombinant mouse ACE2 standard | 10ng/tube×2 |
| EK1188-DA | Biotinylated anti- mouse ACE2 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 146 | 538 | 1252 | 131 | 557 | 1192 |
| Standard deviation | 11.38 | 25.28 | 76.37 | 10.48 | 31.74 | 84.63 |
| CV(%) | 7.8% | 4.7% | 6.1% | 8% | 5.7% | 7.1% |
Mouse ACE2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.055 | 0.091 | 0.132 | 0.226 | 0.412 | 0.730 | 1.331 | 2.160 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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