产品概况
货号 | EK1443 |
---|---|
产品名称 | Mouse BMP7 ELISA Kit |
产品描述 | For quantitative detection of mouse BMP-7 in bone tissue, , serum and plasma((heparin, EDTA). |
免疫原 | CHO, S292-H430 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | BMP7 |
基因全名 | bone morphogenetic protein 7 |
Uniprot ID | P23359 |
指标别名 | BMP 7|BMP7|BMP-7|Bone morphogenetic protein 7|Eptotermin alfa|OP 1|OP1|OP1-|Osteogenic protein 1 |
背景资料 | Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1443-CAP | 96-well plate precoated with anti- mouse BMP-7 antibody | 1 |
EK1443-ST | Lyophilized recombinant mouse BMP-7 standard | 10ng/tube×2 |
EK1443-DA | Biotinylated anti- mouse BMP-7 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 82 | 219 | 964 | 87 | 198 | 895 |
Standard deviation | 4.83 | 14.45 | 74.22 | 6.78 | 13.46 | 84.13 |
CV(%) | 5.9% | 6.6% | 7.7% | 7.8% | 6.8% | 9.4% |
Mouse BMP7 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.032 | 0.085 | 0.143 | 0.248 | 0.423 | 0.816 | 1.359 | 2.231 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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