产品简介

RNasin Ribonuclease Inhibitors have broad-spectrum RNase inhibitory properties, including inhibition of eukaryotic RNases of the neutral type. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at a 1:1 ratio. The Ki value for the binding of RNasin Ribonuclease Inhibitor to RNase (e.g., RNase A) is approximately 10-14M. In addition, the kinetics of association for RNasin Ribonuclease Inhibitor is very rapid, ensuring immediate complexing and inhibition of RNase. RNasin Ribonuclease Inhibitors is purified using a combination of ion exchange and affinity chromatography. They are devoid of DNA exonuclease and endonuclease activity and RNase activity. Isolated from a recombinant E. coli strain, the N-terminus is an unblocked serine residue.

单位定义

One unit is defined as the amount of RNasin Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2´,3´-cyclic monophosphate by ribonuclease A.