货号 | EK0443 |
---|---|
产品名称 | Human MCP-3 ELISA Kit |
产品描述 | For quantitative detection of human CCL7 in , cell lysates |
免疫原 | E.coli, Q24-L99 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | CCL7 |
基因全名 | chemokine (C-C motif) ligand 7 |
Uniprot ID | P80098 |
指标别名 | C-C motif chemokine 7|Ccl7|CCL7_HUMAN|Chemokine CC Motif Ligand 7|Chemotactic protein 3|FIC|Intercrine/chemokine MARC|MARC|MCP 3|MCP-3|Monocyte chemoattractant protein 3|Monocyte chemotactic protein 3|NC28|RANTES/sis homolog|RP23-350G1.4|SCYA6|SCYA7|Small inducible cytokine A7|Small-inducible cytokine A7 |
背景资料 | Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0443-CAP | 96-well plate precoated with anti- human CCL7 antibody | 1 |
EK0443-ST | Lyophilized recombinant human CCL7 standard | 10ng/tubex2 |
EK0443-DA | Biotinylated anti- human CCL7 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 38 | 169 | 505 | 37 | 158 | 504 |
Standard deviation | 2.39 | 12.67 | 27.27 | 2.55 | 12.79 | 31.75 |
CV(%) | 6.3% | 7.5% | 5.4% | 6.9% | 8.1% | 6.3% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.003 | 0.088 | 0.194 | 0.424 | 0.885 | 1.454 | 1.991 | 2.295 |
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