货号 | BM1583 |
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产品名称 | Anti-CCND2 Antibody(Monoclonal, DCS-3) |
基因名 | CCND2 |
抗体来源 | Mouse |
克隆 | Monoclonal (Clone: DCS-3) |
抗体亚型 | Mouse IgG2a |
分子量 | 33KD |
免疫原 | Recombinant human cyclin D2 protein. |
内容 | 200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol. |
纯化方式 | Ascites |
浓度 | 200ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Inaba et al.(1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2(CCND2) gene ia assigned to 12p13. |
研究类别 | 1. Kozar, K.; Ciemerych, M. A.; Rebel, V. I.; Shigematsu, H.; Zagozdzon, A.; Sicinska, E.; Geng, Y.; Yu, Q.; Bhattacharya, S.; Bronson, R. T.; Akashi, K.; Sicinski, P. : Mouse development and cell proliferation in the absence of D-cyclins. Cell 118: 477-491, 2004. 2. Inaba, T.; Matsushime, H.; Valentine, M.; Roussel, M. F.; Sherr, C. J.; Look, A. T. : Genomic organization, chromosomal localization, and independent expression of human cyclin D genes. Genomics 13: 565-574, 1992. 3. Xiong, Y.; Menninger, J.; Beach, D.; Ward, D. C. : Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics 13: 575-584, 1992. |
Uniprot ID | CCND2: P30279 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot: | 1:500-2000 |
Immunocytochemistry: | 1:50-400 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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