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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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通用实验耗材
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色谱耗材
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产品概况
| 货号 | BM0771 |
|---|---|
| 产品名称 | Anti-CCND1 Antibody(Monoclonal, DCS-6) |
| 基因名 | CCND1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal (Clone: DCS-6) |
| 抗体亚型 | Mouse IgG2a |
| 分子量 | 33KD |
| 免疫原 | Recombinant human cyclin D1 protein. |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA |
| 纯化方式 | Ascites |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1(CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein. |
| 研究类别 | 1. Rosenberg, C. L.; Kim, H. G.; Shows, T. B.; Kronenberg, H. M.; Arnold, A. : Rearrangement and overexpression of D11S287E, a candidate oncogene on chromosome 11q13 in benign parathyroid tumors. Oncogene 6: 449-453, 1991. 2. Muller, H.; Lukas, J.; Schneider, A.; Warthoe, P.; Bartek, J.; Eilers, M.; Strauss, M. : Cyclin D1 expression is regulated by the retinoblastoma protein. Proc. Nat. Acad. Sci. 91: 2945-2949, 1994. 3. Hinds, P. W.; Dowdy, S. F.; Eaton, E. N.; Arnold, A.; Weinberg, R. A. : Function of a human cyclin gene as an oncogene. Proc. Nat. Acad. Sci. 91: 709-713, 1994. |
| Uniprot ID | CCND1: Human(P24385), Mouse(P25322), Rat(P39948) |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot: | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Immunohistochemistry(Frozen Section): | 1:50-400 |
| Immunocytochemistry: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大 [list_product_images]IHC(F): Mouse Intestine Tissue|IHC(F): Mouse Spleen Tissue|IHC(F): Rat Intestine Tissue|IHC(F): Human Placenta Tissue|Figure 5. Western blot analysis of anti- CCND1 antibody (BM0771).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: Mouse brain tissue lysates,Lane 3: human hela whole cell lysates,Use rabbit anti- CCND1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CCND1 at approximately 33KD. The expected band size for CCND1 is at 33KD.[/list_product_images]
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