Mouse ALCAM ELISA Kit

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货号：EK1184

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1184-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1184
产品名称	Mouse ALCAM ELISA Kit
产品描述	For quantitative detection of mouse ALCAM in , cell lysates
免疫原	NSO, W28-K527
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	78.1pg/ml-5000pg/ml
基因名称	ALCAM
基因全名	activated leukocyte cell adhesion molecule
Uniprot ID	Q61490
指标别名	ALCAM protein \| CD166 antigen \| Protein MEMD \| Q13740
背景资料	CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the Chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
显色时间	25-30分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1184-CAP	96-well plate precoated with anti- mouse ALCAM antibody	1
EK1184-ST	Lyophilized recombinant mouse ALCAM standard	10ng/tube×2
EK1184-DA	Biotinylated anti- mouse ALCAM antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse ALCAM ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 25-30分钟)

Concentration(pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.066	0.098	0.164	0.294	0.544	1.007	1.821	2.388

*典型数据仅供参考。用户需要自己建立标准曲线。

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