特点

•     · In-unit digestion saves time and samples
•     · Column-based purification protocol eliminates the need for harsh chemicals
•     · Fast protocol takes only 40 minutes

产品详情

Removing important sample data from Millipore’s Sterivex filter units present a unique set of problems for researchers, however the DNeasy PowerWater Sterivex Kit is the only kit available to provide DNA from these filters without enzymes or harsh organic chemicals. Microbes are released from Sterivex filter units utilizing a novel Cell Release Solution that eliminates extensive incubation times or breaking the casing around the membrane. With Inhibitor Removal Technology, high-quality DNA isolation is possible, even when the original sample was highly contaminated. In 40 minutes, DNA can be purified from all types of water samples.
This kit is recommended for use with Millipore catalog # SVGPL10RC Sterivex GP Filter units, but is also compatible with other Sterivex Filter units.

DNeasy PowerWater Sterivex Kit 之前由MO BIO作为 PowerWater Sterivex DNA Kit 出售。

性能

To begin the DNeasy PowerWater Sterivex Kit protocol, Sterivex units are treated and incubated with cell release solution. Units are then treated with lysis buffer, followed by mixing and removing lysate for homogenization in a 5 ml bead beating tube. Total genomic DNA is captured after protein and inhibitor removal steps on a silica binding column using a vacuum manifold. The binding column membrane is then washed, resulting in pure, high-quality DNA being eluted for downstream applications.