Human CCL8/MCP-2 ELISA Kit

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货号：EK0442

反应性：Human

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0442-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0442
产品名称	Human MCP-2 ELISA Kit
产品描述	For quantitative detection of human CCL8 in , cell lysates
免疫原	E.coli, Q24-P99
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<1pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	CCL8
基因全名	chemokine (C-C motif) ligand 8
Uniprot ID	P80075
指标别名	C C motif chemokine 8\|CCL 8\|Ccl8\|CCL8_HUMAN\|Chemokine (C C motif) ligand 8\|Chemokine ligand 8\|HC14\|MCP 2\|MCP-2\|MCP-2(6-76)\|Monocyte chemoattractant protein 2\|Monocyte chemotactic protein 2\|SCYA10\|SCYA8\|Small inducible cytokine A8\|Small inducible cytokine subfamily A (Cys Cys) member 8\|Small-inducible cytokine A8
背景资料	Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
显色时间	15-20分钟

SKU	名称	数量
Catalog number	Description	Quantity
EK0442-CAP	96-well plate precoated with anti- human CCL8 antibody	1
EK0442-ST	Lyophilized recombinant human CCL8 standard	10ng/tubex2
EK0442-DA	Biotinylated anti- human CCL8 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.001	0.054	0.087	0.199	0.500	0.905	1.927	2.045

*典型数据仅供参考。用户需要自己建立标准曲线。

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