Rat Activin A ELISA Kit

货号:EK0303
反应性:Rat
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产品概况

货号EK0303
产品名称Rat Activin A ELISA Kit
产品描述For quantitative detection of rat Activin A in , serum, plasma(heparin, EDTA) and saliva.
免疫原CHO, G311-S426
交叉反应There is no detectable cross-reactivity with other relevant proteins.
敏感性<12pg/ml
检测范围15.6pg/ml-1000pg/ml
基因名称INHBA
基因全名inhibin, beta A
Uniprot IDP18331
指标别名Activin beta A chain|erythroid differentiation factor|erythroid differentiation protein|follicle stimulating hormone releasing protein|FSH releasing protein|Inhibin|inhibin beta A chain
背景资料Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
显色时间25-30分钟

试剂盒内容

SKU名称数量
Catalog numberDescriptionQuantity
EK0303-CAP96-well plate precoated with anti- rat Activin A antibody1
EK0303-STLyophilized recombinant rat Activin A standard10ng/tube×2
EK0303-DABiotinylated anti- rat Activin A antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

  • 1.96孔读板仪器。
  • 2.自动洗板机.
  • 3.移液器和枪头。建议使用多通道移液器/排枪。
  • 4.Eppendorf 管。
  • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3115145028138453
Standard deviation1.3825.21.568.6925.36
CV(%)4.2%5.3%5.6%5.6%6.3%5.6%

Rat Activin A ELISA Kit的典型数据示例(取自最近的一批次质检数据)

(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0280.0490.1110.1750.3280.8511.4572.491
*典型数据仅供参考。用户需要自己建立标准曲线。
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