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产品概况
| 货号 | EK0851 |
|---|---|
| 产品名称 | Human CD10/Neprilysin ELISA Kit |
| 产品描述 | For quantitative detection of human CD10 in , cell lysates |
| 免疫原 | CHO, Y52-W750 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 125pg/ml-8000pg/ml |
| 基因名称 | MME |
| 基因全名 | membrane metallo-endopeptidase |
| Uniprot ID | P08473 |
| 指标别名 | Atriopeptidase|CALLA|CD 10|CD10|CD10 antigen|Common acute lymphocytic leukemia antigen|DKFZp686O16152|EC 3.4.24.11|Enkephalinase|EPN|gp100|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase)|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)|Membrane metallo endopeptidase|Membrane metallo endopeptidase variant 1|Membrane metallo endopeptidase variant 2|Membrane metalloendopeptidase|Membrane metalloendopeptidase neutral endopeptidase enkephalinase|Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10|Membrane metalloendopeptidase variant 1|Membrane metalloendopeptidase variant 2|MGC126681|MGC126707|MME|NEP|NEP_HUMAN|Neprilysin|Neutral endopeptidase 24.11|Neutral endopeptidase|SFE|Skin fibroblast elastase |
| 背景资料 | CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0851-CAP | 96-well plate precoated with anti- human CD10 antibody | 1 |
| EK0851-ST | Lyophilized recombinant human CD10 standard | 10ng/tube×2 |
| EK0851-DA | Biotinylated anti- human CD10 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 201 | 956 | 4316 | 216 | 881 | 4302 |
| Standard deviation | 14.87 | 51.62 | 233.06 | 17.92 | 60.78 | 305.44 |
| CV(%) | 7.4% | 5.4% | 5.4% | 8.3% | 6.9% | 7.1% |
Human CD10/Neprilysin ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)
| Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.097 | 0.148 | 0.196 | 0.319 | 0.462 | 0.858 | 1.328 | 1.965 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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