产品概况
货号 | EK0558 |
---|---|
产品名称 | Mouse ACE ELISA Kit |
产品描述 | For quantitative detection of mouse ACE in , serum and plasma(heparin). |
免疫原 | NSO, L35-Q1264 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 93.8pg/ml-6000pg/ml |
基因名称 | ACE |
基因全名 | angiotensin I converting enzyme |
Uniprot ID | P09470 |
指标别名 | ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A |
背景资料 | Angiotensin-converting enzyme(ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system(RAS), which mediates extracellular volume(i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0558-CAP | 96-well plate precoated with anti- mouse ACE antibody | 1 |
EK0558-ST | Lyophilized recombinant mouse ACE standard | 10ng/tubex2 |
EK0558-DA | Biotinylated anti- mouse ACE antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 157 | 733 | 3455 | 155 | 795 | 3149 |
Standard deviation | 6.9 | 40.31 | 272.94 | 7.59 | 46.11 | 286.55 |
CV(%) | 4.4% | 5.5% | 7.9% | 4.9% | 5.8% | 9.1% |
Mouse ACE ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.084 | 0.128 | 0.178 | 0.253 | 0.444 | 0.658 | 1.511 | 2.327 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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