Rat Adiponectin ELISA Kit

货号:EK1327
反应性:Rat
SKU 包装规格 单位 目录价 因厂家不定期调价可能造成价格未能及时更新,具体价格以下单时客服确认为准 您的价格 注册会员请联系客服或销售申请品牌专属折扣 库存 数量 购物车
EK1327-96T 96T EA ¥2480.00 请登录 请登录

产品概况

货号 EK1327
产品名称 Rat Adiponectin ELISA Kit
产品描述 For quantitative detection of rat Adiponectin in , serum, plasma(heparin, EDTA)and urine.
免疫原 E.coli, I19-N244
交叉反应 There is no detectable cross-reactivity with other relevant proteins.
敏感性 <10pg/ml
检测范围 156pg/ml-10000pg/ml
基因名称 ADIPOQ
基因全名 adiponectin, C1Q and collagen domain containing
Uniprot ID Q8K3R4
指标别名 30 kDa adipocyte complement related protein|30 kDa adipocyte complement-related protein|ACDC|ACRP30|ADIPO_HUMAN|Adipocyte|Adipocyte C1q and collagen domain containing protein|Adipocyte complement related 30 kDa protein|Adipocyte complement related protein of 30 kDa|Adipocyte complement-related 30 kDa protein|Adiponectin|Adipoq|Adipose most abundant gene transcript 1|Adipose most abundant gene transcript 1 protein|Adipose specific collagen like factor|ADIPQTL1|ADPN|apM-1|APM1|C1q and collagen domain-containing protein|GBP28|Gelatin binding protein|Gelatin binding protein 28|Gelatin-binding protein
背景资料 Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
显色时间 25-30 分钟

试剂盒内容

SKU 名称 数量
Catalog number Description Quantity
EK1327-CAP 96-well plate precoated with anti- rat Adiponectin antibody 1
EK1327-ST Lyophilized recombinant rat Adiponectin standard 10ng/tube×2
EK1327-DA Biotinylated anti- rat Adiponectin antibody 100μl(dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 100μl(dilution 1:100)
AR1106-1 Sample diluent buffer 30ml
AR1106-2 Antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

  • 1.96孔读板仪器。
  • 2.自动洗板机.
  • 3.移液器和枪头。建议使用多通道移液器/排枪。
  • 4.Eppendorf 管。
  • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 292 1705 4269 265 1626 4075
Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07
CV(%) 5.8% 7.4% 4% 7.4% 8.2% 4.1%

Rat Adiponectin ELISA Kit的典型数据示例(取自最近的一批次质检数据)

(TMB 孵育条件: 37°C, 25-30 分钟)

Concentration(pg/ml) 0 156 312 625 1250 2500 5000 10000
O.D. 0.004 0.086 0.164 0.305 0.588 0.961 1.511 1.937

*典型数据仅供参考。用户需要自己建立标准曲线。

暂无产品信息
暂无参考文献
暂无文件下载