Rat CCL7/MCP-3 ELISA Kit

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货号：EK1300

反应性：Rat

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1300-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1300
产品名称	Rat MCP-3 ELISA Kit
产品描述	For quantitative detection of rat CCL7 in , cell lysates
免疫原	E.coli, Q24-P97
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	CCL7
基因全名	chemokine (C-C motif) ligand 7
Uniprot ID	Q9QXY8
指标别名	C-C motif chemokine 7\|Ccl7\|CCL7_HUMAN\|Chemokine CC Motif Ligand 7\|Chemotactic protein 3\|FIC\|Intercrine/chemokine MARC\|MARC\|MCP 3\|MCP-3\|Monocyte chemoattractant protein 3\|Monocyte chemotactic protein 3\|NC28\|RANTES\|sis homolog\|RP23-350G1.4\|SCYA6\|SCYA7\|Small inducible cytokine A7\|Small-inducible cytokine A7
背景资料	Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
显色时间	25-30分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1300-CAP	96-well plate precoated with anti- rat CCL7 antibody	1
EK1300-ST	Lyophilized recombinant rat CCL7 standard	10ng/tube×2
EK1300-DA	Biotinylated anti- rat CCL7 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Rat MCP-3 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 25-30分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.010	0.087	0.165	0.324	0.617	1.144	1.938	2.563

*典型数据仅供参考。用户需要自己建立标准曲线。

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