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产品概况
| 货号 | EK0446 |
|---|---|
| 产品名称 | Human MDC ELISA Kit |
| 产品描述 | For quantitative detection of human MDC in , cell lysates |
| 免疫原 | E.coli, G25-Q93 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | CCL22 |
| 基因全名 | chemokine (C-C motif) ligand 22 |
| Uniprot ID | O00626 |
| 指标别名 | A-152E5.1|ABCD 1|ABCD1|C-C motif chemokine 22|CC chemokine STCP-1|Ccl22|CCL22_HUMAN|Chemokine (C-C motif) ligand 22|DC/B-CK|Macrophage-derived chemokine|MDC(1-69)|MDC(7-69)|MGC34554|SCYA22|Small inducible cytokine A22|Small inducible cytokine subfamily A (Cys-Cys), member 22|Small-inducible cytokine A22|STCP 1|STCP1|Stimulated T cell chemotactic protein 1|Stimulated T-cell chemotactic protein 1 |
| 背景资料 | Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0446-CAP | 96-well plate precoated with anti- human MDC antibody | 1 |
| EK0446-ST | Lyophilized recombinant human MDC standard | 10ng/tubex2 |
| EK0446-DA | Biotinylated anti- human MDC antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 97 | 212 | 842 | 87 | 210 | 866 |
| Standard deviation | 7.37 | 9.11 | 55.57 | 7.91 | 12.6 | 64.08 |
| CV(%) | 7.6% | 4.3% | 6.6% | 9.1% | 6% | 7.4% |
Human MDC ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.003 | 0.056 | 0.120 | 0.256 | 0.508 | 0.903 | 1.488 | 1.957 |
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