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品名：Human CD25/IL-2R alpha ELISA Kit
品牌：博士德
货号：EK0400 -
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产品概况
| 货号 | EK0400 |
|---|---|
| 产品名称 | Human CD25/IL-2R alpha ELISA Kit |
| 产品描述 | For quantitative detection of human CD25 in , cell lysates |
| 免疫原 | NSO,E22-C213 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <5pg/ml |
| 检测范围 | 78pg/ml-5000pg/ml |
| 基因名称 | IL2RA |
| 基因全名 | interleukin 2 receptor, alpha |
| Uniprot ID | P01589 |
| 指标别名 | CD122|CD122 antigen|CD132|CD132 antigen|CD25|CD25 antigen|Cytokine receptor common gamma chain|Gamma C|High affinity IL 2 receptor subunit beta|IDDM10|IL 2 RA|IL 2 receptor|IL 2 receptor alpha subunit|IL 2R gamma chain|IL2 RA|IL2R|IL2RA|IL2RB|IL2RG|IMD4|interleukin 2 receptor alpha|Interleukin 2 receptor alpha chain|interleukin 2 receptor beta|Interleukin 2 receptor beta chain|interleukin 2 receptor gamma|Interleukin 2 receptor gamma (severe combined immunodeficiency)|Interleukin 2 receptor gamma chain|p55|p64|P70 75|p75|SCIDX|SCIDX1|TAC antigen|TCGFR|XSCID |
| 背景资料 | CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0400-CAP | 96-well plate precoated with anti- human CD25 antibody | 1 |
| EK0400-ST | Lyophilized recombinant human CD25 standard | 10ng/tubex2 |
| EK0400-DA | Biotinylated anti- human CD25 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 140 | 719 | 2353 | 141 | 785 | 2310 |
| Standard deviation | 6.58 | 41.7 | 94.12 | 7.61 | 47.1 | 97.02 |
| CV(%) | 4.7% | 5.8% | 4% | 5.4% | 6% | 4.2% |
Human CD25/IL-2R alpha ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.016 | 0.251 | 0.435 | 0.770 | 1.108 | 1.479 | 1.853 | 2.049 |
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