Human AFP ELISA Kit

产品中心

浏览历史

货号：EK0651

反应性：Human

说明书下载收藏产品大包装咨询？

SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0651-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0651
产品名称	Human AFP ELISA Kit
产品描述	For quantitative detection of human AFP in , cell lysates
免疫原	NSO, R19-V609
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	78pg/ml-5000pg/ml
基因名称	AFP
基因全名	alpha fetoprotein
Uniprot ID	P02771
指标别名	Afp\|Alpha fetoglobulin\|Alpha-fetoglobulin\|Alpha fetoprotein\|Alpha-fetoprotein\|Alpha-1-fetoprotein\|FETA\|HPAFP
背景资料	Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0651-CAP	96-well plate precoated with anti- human AFP antibody	1
EK0651-ST	Lyophilized recombinant human AFP standard	5ng/tube×2
EK0651-DA	Biotinylated anti- human AFP antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Human AFP ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.003	0.137	0.248	0.486	0.777	1.260	1.704	1.925

*典型数据仅供参考。用户需要自己建立标准曲线。

暂无产品信息

暂无参考文献

暂无文件下载