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产品概况
| 货号 | EK1128 |
|---|---|
| 产品名称 | Mouse CCL12 ELISA Kit |
| 产品描述 | For quantitative detection of mouse CCL12 in , cell lysates |
| 免疫原 | E.coli, G23-G104 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml→1000pg/ml |
| 基因名称 | Ccl12 |
| 基因全名 | chemokine (C-C motif) ligand 12 |
| Uniprot ID | Q62401 |
| 指标别名 | C C motif chemokine 12|Ccl12|MCP 1 related chemokine|Monocyte chemoattractant protein 5|Monocyte chemotactic protein 5|Scya12|Small inducible cytokine A12 |
| 背景资料 | Chemokine(C-C motif) ligand 12(CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5(MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11. |
| 显色时间 | 15-20 分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1128-CAP | 96-well plate precoated with anti- mouse CCL12 antibody | 1 |
| EK1128-ST | Lyophilized recombinant mouse CCL12 standard | 10ng/tube×2 |
| EK1128-DA | Biotinylated anti- mouse CCL12 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 88 | 249 | 906 | 82 | 260 | 979 |
| Standard deviation | 6.6 | 18.67 | 44.39 | 7.38 | 21.58 | 63.63 |
| CV(%) | 7.5% | 7.5% | 4.9% | 9% | 8.3% | 6.5% |
Mouse CCL12 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20 分钟)
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.031 | 0.074 | 0.157 | 0.305 | 0.587 | 1.032 | 1.901 | 2.369 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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