Mouse CD30L ELISA Kit

产品中心

浏览历史

货号：EK0572

反应性：Mouse

说明书下载收藏产品大包装咨询？

SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0572-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0572
产品名称	Mouse CD30L ELISA Kit
产品描述	For quantitative detection of mouse CD30L in , cell lysates
免疫原	NSO, Q68-D239
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<6pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	TNFSF8
基因全名	tumor necrosis factor (ligand) superfamily, member 8
Uniprot ID	P32972
指标别名	CD153 antigen\|CD30 antigen ligand\|CD30 L\|CD30 ligand\|CD30L\|CD30LG\|MGC138144\|TNFSF8\|Tumor necrosis factor (ligand) superfamily member 8\|Tumor necrosis factor ligand superfamily member 8
背景资料	The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154(CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.
显色时间	25-30分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0572-CAP	96-well plate precoated with anti- mouse CD30L antibody	1
EK0572-ST	Lyophilized recombinant mouse CD30L standard	10ng/tubex2
EK0572-DA	Biotinylated anti- mouse CD30L antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CD30L ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 25-30分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.073	0.112	0.133	0.203	0.326	0.594	1.146	2.091

*典型数据仅供参考。用户需要自己建立标准曲线。

暂无产品信息

暂无参考文献

暂无文件下载