产品概况
货号 | EK0978 |
---|---|
产品名称 | Rat CRP ELISA Kit |
产品描述 | For quantitative detection of rat CRP in , cell lysates |
免疫原 | NSO, H20-S230 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CRP |
基因全名 | C-reactive protein, pentraxin-related |
Uniprot ID | P48199 |
指标别名 | C reactive protein pentraxin related|C-reactive protein|C-reactive protein(1-205)|CRP|CRP_HUMAN|MGC149895|MGC88244|Pentraxin 1|Pentraxin 1 short|PTX 1|PTX1 |
背景资料 | C Reactive Protein(CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0978-CAP | 96-well plate precoated with anti- rat CRP antibody | 1 |
EK0978-ST | Lyophilized recombinant rat CRP standard | 10ng/tube×2 |
EK0978-DA | Biotinylated anti- rat CRP antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 225 | 1505 | 4812 | 235 | 1505 | 4457 |
Standard deviation | 11.02 | 81.27 | 307.96 | 11.75 | 108.36 | 329.81 |
CV(%) | 4.9% | 5.4% | 6.4% | 5% | 7.2% | 7.4% |
Rat CRP ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.033 | 0.055 | 0.111 | 0.207 | 0.399 | 0.735 | 1.251 | 2.029 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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