产品概况
货号 | EK0912 |
---|---|
产品名称 | Human FCER2/CD23 ELISA Kit |
产品描述 | For quantitative detection of human CD23 in , serum, plasma(heparin, EDTA) and urine. |
免疫原 | NSO, D48-S321 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | FCER2 |
基因全名 | Fc fragment of IgE, low affinity II, receptor for (CD23) |
Uniprot ID | P06734 |
指标别名 | BLAST 2|BLAST-2|C-type lectin domain family 4 member J|CD23|CD23 antigen|CD23A|CLEC4J|Fc fragment of IgE low affinity II receptor for (CD23)|Fc-epsilon-RII|FCE2|Fcer2|FCER2_HUMAN|IGEBF|Immunoglobulin E-binding factor|Low affinity immunoglobulin epsilon Fc receptor|Low affinity immunoglobulin epsilon Fc receptor soluble form|Lymphocyte IgE receptor |
背景资料 | CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23(FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0912-CAP | 96-well plate precoated with anti- human CD23 antibody | 1 |
EK0912-ST | Lyophilized recombinant human CD23 standard | 10ng/tube×2 |
EK0912-DA | Biotinylated anti- human CD23 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 61 | 194 | 981 | 55 | 207 | 899 |
Standard deviation | 2.8 | 7.95 | 64.74 | 3.41 | 9.93 | 71.02 |
CV(%) | 4.6% | 4.1% | 6.6% | 6.2% | 4.8% | 7.9% |
Human FCER2/CD23 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.012 | 0.052 | 0.109 | 0.200 | 0.403 | 0.660 | 1.477 | 2.083 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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