货号 | BM1620 |
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产品名称 | Anti-GSN Antibody |
基因名 | GSN |
抗体来源 | Mouse |
克隆 | Monoclonal (Clone: GS-2C4) |
抗体亚型 | Mouse IgG1 |
分子量 | 86KD |
免疫原 | Human plasma gelsolin. |
内容 | 200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol. |
纯化方式 | Ascites |
浓度 | 200ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. |
研究类别 | 1. Kwiatkowski, D. J.; Ozelius, L.; Schuback, D.; Gusella, J.; Breakefield, X. O. : The gelsolin (GSN) cDNA clone, from 9q32-34, identifies BclI and StuI RFLPs. Nucleic Acids Res. 17: 4425 only, 1989.2. Kwiatkowski, D. J.; Westbrook, C. A.; Bruns, G. A. P.; Morton, C. C. : Localization of gelsolin proximal to ABL on chromosome 9. Am. J. Hum. Genet. 42: 565-572, 1988. 3. Kwiatkowski, D. J.; Stossel, T. P.; Orkin, S. H.; Mole, J. E.; Colten, H. R.; Yin, H. L. : Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature 323: 455-458, 1986. |
Uniprot ID | GSN: Human(P06396), Mouse(P13020), Rat(Q68FP1) |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot: | 1:500-2000 |
Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
Immunohistochemistry(Frozen Section): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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