产品名称:β-Tubulin mouse monoclonal antibody
克隆号:6G4
同种型:IgG1
免疫原:人全长β-Tubulin
测试应用:WB、IHC。尚未在其他应用中测试。
推荐稀释度:
WB 1:2000-5000
IHC 1:200
IF 1:100-200
最佳稀释/浓度应由最终用户确定。
分子量:55KD
形式:液体
浓度:1mg/ml在PBS中,pH 7.4,含0.02%叠氮化钠和50%甘油
储存:β-Tubulin抗体在2-8℃下稳定1个月。对于长期储存,将其等分并储存在-20°C或
在下面避免反复冻融循环。
反应性:H、R、M、Mk、斑马鱼
产品简介
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha-and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with β-tubulin mouse mAb(6G4) diluted at 1:5000.
Immunohistochemistry Staining of Human colon tissue with β-Tubulin mouse mAb(6G4) diluted at 1:200.
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