产品概况
货号 | EK1315 |
---|---|
产品名称 | Human CD21 ELISA Kit |
产品描述 | For quantitative detection of human CD21 in , cell lysates |
免疫原 | CHO, Ile21-71 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <20pg/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | CR2 |
基因全名 | complement component (3d/Epstein Barr virus) receptor 2 |
Uniprot ID | P20023 |
指标别名 | C3DR|CD 21|CD21|CD21 antigen|Complement C3d receptor|Complement component (3d/Epstein Barr virus) receptor 2|Complement component receptor 2|Complement receptor type 2|Cr 2|CR|Cr2|CR2_HUMAN|CVID7|EBV receptor|EBV-R|Epstein Barr virus receptor|Epstein-Barr virus receptor|EVBR|SLEB9 |
背景资料 | Complement receptor type 2(CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). |
显色时间 | 15-20分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1315-CAP | 96-well plate precoated with anti- human CD21 antibody | 1 |
EK1315-ST | Lyophilized recombinant human CD21 standard | 50ng/tube×2 |
EK1315-DA | Biotinylated anti- human CD21 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1242 | 7916 | 24012 | 1177 | 7910 | 25042 |
Standard deviation | 70.79 | 372.05 | 1296.64 | 89.45 | 411.32 | 1627.73 |
CV(%) | 5.7% | 4.7% | 5.4% | 7.6% | 5.2% | 6.5% |
Human CD21 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 15-20分钟)
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.003 | 0.355 | 0.665 | 1.038 | 1.494 | 1.972 | 2.210 | 2.198 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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