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产品概况
| 货号 | EK0331 |
|---|---|
| 产品名称 | Human Eotaxin-2 ELISA Kit |
| 产品描述 | For quantitative detection of human CCL24 in , cell lysates |
| 免疫原 | E.coli, V27-C119 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <2pg/ml |
| 检测范围 | 31.2-2000pg/ml |
| 基因名称 | CCL24 |
| 基因全名 | chemokine (C-C motif) ligand 24 |
| Uniprot ID | O00175 |
| 指标别名 | C C motif chemokine 24|CCL24|Chemokine (C C motif) ligand 24|Chemokine CC Motif Ligand 2|CK beta 6|Ckb 6|Ckb6|Eosinophil chemotactic protein 2|Eotaxin 2 like protein|MPIF 2|MPIF2|Myeloid progenitor inhibitory factor 2|OTTHUMP00000210225|SCYA24|Small inducible cytokine A24|Small inducible cytokine subfamily A (Cys Cys) member 24 |
| 背景资料 | Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0331-CAP | 96-well plate precoated with anti- human CCL24 antibody | 1 |
| EK0331-ST | Lyophilized recombinant human CCL24 standard | 10ng/tube×2 |
| EK0331-DA | Biotinylated anti- human CCL24 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 130 | 767 | 2774 | 135 | 805 | 2889 |
| Standard deviation | 9.23 | 30.68 | 110.96 | 10.8 | 41.86 | 158.89 |
| CV(%) | 7.1% | 4% | 4% | 8% | 5.2% | 5.5% |
Human Eotaxin-2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.040 | 0.222 | 0.377 | 0.684 | 1.159 | 1.741 | 1.979 | 2.121 |
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