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产品概况
| 货号 | EK1313 |
|---|---|
| 产品名称 | Mouse CD32/FCGR2b/c ELISA Kit |
| 产品描述 | For quantitative detection of mouse CD32 in , cell lysates |
| 免疫原 | NSO, T40-R217 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | FCGR2B |
| 基因全名 | Fc fragment of IgG, low affinity IIb, receptor (CD32) |
| Uniprot ID | P08101 |
| 指标别名 | CD 32|CD32|CD32 antigen|CD32A|CD32B|CD32C|CDw32|Fc fragment of IgG low affinity II receptor for (CD32)|Fc fragment of IgG low affinity IIa receptor|Fc fragment of IgG low affinity IIa receptor for (CD32)|Fc fragment of IgG low affinity IIb receptor|Fc fragment of IgG low affinity IIb receptor for (CD32)|Fc fragment of IgG low affinity IIc receptor|Fc fragment of IgG low affinity IIc receptor for (CD32)|Fc gamma receptor IIB|Fc gamma RII a|Fc gamma RII b|Fc gamma RII c|Fc gamma RIIa|Fc gamma RIIb |Fc gamma RIIc|Fc receptor, IgG, low affinity IIb|Fc-gamma RII-a|Fc-gamma RII-b|Fc-gamma RII-c|Fc-gamma-RIIa|FC-gamma-RIIB|Fc-GAMMA-RIIC|Fc[g]RII|FCG 2|FCG2|FCG2A_HUMAN|FcgammaRIIC|FCGR 2|FcGR|FCGR2|FCGR2A|FCGR2A1|FCGR2B|FCGR2C|Fcgr3|Fcgr3a|FcgRII|Fcr-2|Fcr-3|FcRII a|FCRII|FcRII b|FcRII c|FcRII-a|FcRIIC|IGFR 2|IGFR2|IgG Fc receptor II a|IgG Fc receptor II b|IgG Fc receptor II beta|IgG Fc receptor II c|IgG Fc receptor II-a|IgG Fc receptor II-b|IgG Fc receptor II-c|Immunoglobulin G Fc receptor II|Low affinity|immunoglobulin gamma Fc region receptor II a|Low affinity immunoglobulin gamma Fc region receptor II|Low affinity immunoglobulin gamma Fc region receptor II b|Low affinity immunoglobulin gamma Fc region receptor II c|Low affinity immunoglobulin gamma Fc region receptor II-a|Low affinity immunoglobulin gamma Fc region receptor II-b|Low affinity immunoglobulin gamma Fc region receptor II-c|Ly-17|Ly-m20|LyM-1|Lymphocyte antigen 17|MGC23887|MGC30032 |
| 背景资料 | CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1313-CAP | 96-well plate precoated with anti- mouse CD32 antibody | 1 |
| EK1313-ST | Lyophilized recombinant mouse CD32 standard | 10ng/tube×2 |
| EK1313-DA | Biotinylated anti- mouse CD32 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 89 | 216 | 932 | 94 | 236 | 885 |
| Standard deviation | 5.25 | 16.63 | 46.6 | 6.95 | 20.06 | 46.9 |
| CV(%) | 5.9% | 7.7% | 5% | 7.4% | 8.5% | 5.3% |
Mouse CD32/FCGR2b/c ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.019 | 0.026 | 0.056 | 0.099 | 0.231 | 0.542 | 1.350 | 2.536 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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