Rat Clusterin ELISA Kit

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货号：EK1615

反应性：Rat

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1615-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1615
产品名称	Rat clusterin ELISA Kit
产品描述	For Quantitative Detection of rat Clusterin in
免疫原	NSO, D22-R226 (β) & S227-E448 (α)
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<15pg/ml
检测范围	312pg/ml-20000pg/ml
基因名称	Clu
基因全名	clusterin
Uniprot ID	P05371
指标别名	Clusterin\|Dimeric acid glycoprotein\|DAG\|Sulfated glycoprotein 2\|SGP-2\|Testosterone repressed prostate message 2\|TRPM-2\|Clu
背景资料	Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1615-CAP	96-well plate precoated with anti- rat Clusterin antibody	1
EK1615-ST	Lyophilized recombinant rat Clusterin standard	10ng/tube×2
EK1615-DA	Biotinylated anti- rat Clusterin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Rat clusterin ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20分钟)

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.007	0.082	0.147	0.264	0.521	0.984	1.692	2.555

*典型数据仅供参考。用户需要自己建立标准曲线。

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