产品概况
货号 | EK1146 |
---|---|
产品名称 | Mouse CD163 ELISA Kit |
产品描述 | For quantitative detection of mouse CD163 in , serum and plasma(heparin). |
免疫原 | NSO, V39-T1045 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <20pg/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | CD163 |
基因全名 | CD163 molecule |
Uniprot ID | Q2VLH6 |
指标别名 | CD_antigen=CD163|CD163|Hemoglobin scavenger receptor|M130|MM130|Scavenger receptor cysteine-rich type 1 protein M130 |
背景资料 | CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1146-CAP | 96-well plate precoated with anti- mouse CD163 antibody | 1 |
EK1146-ST | Lyophilized recombinant mouse CD163 standard | 50ng/tube×2 |
EK1146-DA | Biotinylated anti- mouse CD163 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1576 | 5152 | 22633 | 1548 | 5417 | 22589 |
Standard deviation | 122.92 | 221.53 | 1606.94 | 131.58 | 254.59 | 1784.53 |
CV(%) | 7.8% | 4.3% | 7.1% | 8.5% | 4.7% | 7.9% |
Mouse CD163 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.133 | 0.158 | 0.175 | 0.230 | 0.318 | 0.546 | 0.910 | 1.446 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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