产品概况
货号 | EK0961 |
---|---|
产品名称 | Human cathepsin S ELISA Kit |
产品描述 | For quantitative detection of human Cathepsin S in , cell lysates |
免疫原 | NSO, Q17-I331&S109-I331 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | CTSS |
基因全名 | cathepsin S |
Uniprot ID | P25774 |
指标别名 | CTSS |
背景资料 | Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization(FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0961-CAP | 96-well plate precoated with anti- human Cathepsin S antibody | 1 |
EK0961-ST | Lyophilized recombinant human Cathepsin S standard | 10ng/tube×2 |
EK0961-DA | Biotinylated anti- human Cathepsin S antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 177 | 404 | 1975 | 188 | 415 | 1910 |
Standard deviation | 10.08 | 19.79 | 126.4 | 11.65 | 26.14 | 154.71 |
CV(%) | 5.7% | 4.9% | 6.4% | 6.2% | 6.3% | 8.1% |
Human cathepsin S ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.074 | 0.140 | 0.230 | 0.369 | 0.636 | 1.058 | 1.702 | 2.288 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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