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产品概况
| 货号 | EK0939 |
|---|---|
| 产品名称 | Rat MIP-1β ELISA Kit |
| 产品描述 | For quantitative detection of rat CCL4 in , serum and plasma(heparin, EDTA, citrate). |
| 免疫原 | E.Coli, A24-N92 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | CCL4 |
| 基因全名 | chemokine (C-C motif) ligand 4 |
| Uniprot ID | P50230 |
| 指标别名 | ACT-2|ACT2|AT744.1|C-C motif chemokine 4|CC chemokine ligand 4|CCL4|CCL4_HUMAN|G-26 T-lymphocyte-secreted protein|HC21|LAG-1|LAG1|Lymphocyte activation gene 1 protein|Macrophage inflammatory protein 1-beta|MIP-1-beta|MIP-1-beta(1-69)|MIP-1-beta(3-69)|MIP1-B|MIP1B|MIP1B1|PAT 744|Protein H400|SCYA2|SCYA4|SIS-gamma|Small-inducible cytokine A4|T-cell activation protein 2 |
| 背景资料 | Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines(11, 14). |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0939-CAP | 96-well plate precoated with anti- rat CCL4 antibody | 1 |
| EK0939-ST | Lyophilized recombinant rat CCL4 standard | 10ng/tube×2 |
| EK0939-DA | Biotinylated anti- rat CCL4 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 31 | 144 | 453 | 31 | 148 | 419 |
| Standard deviation | 1.42 | 9.21 | 24.91 | 1.67 | 12.13 | 24.3 |
| CV(%) | 4.6% | 6.4% | 5.5% | 5.4% | 8.2% | 5.8% |
Rat MIP-1β ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.005 | 0.112 | 0.234 | 0.450 | 0.835 | 1.179 | 1.667 | 2.174 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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