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产品概况
| 货号 | EK1519 |
|---|---|
| 产品名称 | Mouse ALK-1 ELISA Kit |
| 产品描述 | For quantitative detection of mouse ALK-1 in , cell lysates |
| 免疫原 | NSO, D23-P119 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <15pg/ml |
| 检测范围 | 46.9pg/ml-3000pg/ml |
| 基因名称 | ACVRL1 |
| 基因全名 | activin A receptor like type 1 |
| Uniprot ID | Q61288 |
| 指标别名 | Activin A receptor | ACVRL1 | ACVRLK1 | ALK-1 | ALK1 | ALK 1 | HHT | HHT2 | ORW2 | SKR3 | TSR-I | TSRI | Q61288 |
| 背景资料 | Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1519-CAP | 96-well plate precoated with anti- mouse ALK-1 antibody | 1 |
| EK1519-ST | Lyophilized recombinant mouse ALK-1 standard | 10ng/tube×2 |
| EK1519-DA | Biotinylated anti- mouse ALK-1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 92 | 338 | 1289 | 84 | 319 | 1165 |
| Standard deviation | 6.8 | 23.66 | 101.83 | 6.63 | 27.43 | 93.2 |
| CV(%) | 7.4% | 7% | 7.9% | 7.9% | 8.6% | 8% |
Mouse ALK-1 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)
| Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.032 | 0.060 | 0.138 | 0.239 | 0.538 | 1.045 | 1.895 | 2.280 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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