产品概况
货号 | EK0809 |
---|---|
产品名称 | Human MCP-4 ELISA Kit |
产品描述 | For quantitative detection of human CCL13 in , cell lysates |
免疫原 | E.coli, Q24-T98 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 7.8pg/ml-500pg/ml |
基因名称 | CCL13 |
基因全名 | chemokine (C-C motif) ligand 13 |
Uniprot ID | Q99616 |
指标别名 | C-C motif chemokine 13|CCL13|CCL13_HUMAN|Chemokine (C C motif) ligand 13|CK beta 10|CK-beta-10|CKb10|Macrophage/monocyte chemotactic protein 4|MCP-4|MGC17134|Monocyte chemoattractant protein 4|Monocyte chemotactic protein 4|NCC 1|NCC-1|NCC1|New CC chemokine 1|SCYA13|SCYL1|short chain|Small inducible cytokine A13|Small inducible cytokine A13 precursor|Small inducible cytokine subfamily A (Cys Cys) member 13|Small-inducible cytokine A13 |
背景资料 | Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0809-CAP | 96-well plate precoated with anti- human CCL13 antibody | 1 |
EK0809-ST | Lyophilized recombinant human CCL13 standard | 10ng/tube×2 |
EK0809-DA | Biotinylated anti- human CCL13 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 19 | 85 | 256 | 18 | 88 | 273 |
Standard deviation | 1.14 | 5.1 | 13.05 | 1.26 | 5.63 | 19.11 |
CV(%) | 6% | 6% | 5.1% | 7% | 6.4% | 7% |
Human MCP-4 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.004 | 0.025 | 0.054 | 0.116 | 0.254 | 0.636 | 1.469 | 2.245 |
*典型数据仅供参考。用户需要自己建立标准曲线。
Copyright © 2021 新疆宝信源柏生物技术有限公司 版权所有!
新ICP备2022000265号-1 新公网安备 65010502000866号