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产品概况
| 货号 | EK0671 |
|---|---|
| 产品名称 | Mouse cathepsin B ELISA Kit |
| 产品描述 | For quantitative detection of mouse Cathepsin B in , cell lysates |
| 免疫原 | NSO, H18-F339 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <5pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | CTSB |
| 基因全名 | cathepsin B |
| Uniprot ID | P10605 |
| 指标别名 | aAmyloid precursor protein secretase|APP secretase|APPS|CATB_HUMAN|Cathepsin B heavy chain|Cathepsin B1|CPSB|CTSB|Cysteine protease |
| 背景资料 | Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0671-CAP | 96-well plate precoated with anti- mouse Cathepsin B antibody | 1 |
| EK0671-ST | Lyophilized recombinant mouse Cathepsin B standard | 10ng/tubex2 |
| EK0671-DA | Biotinylated anti- mouse Cathepsin B antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 242 | 1828 | 4749 | 229 | 1916 | 4534 |
| Standard deviation | 14.76 | 100.54 | 370.42 | 13.96 | 128.37 | 439.79 |
| CV(%) | 6.1% | 5.5% | 7.8% | 6.1% | 6.7% | 9.7% |
Mouse cathepsin B ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.021 | 0.126 | 0.233 | 0.379 | 0.665 | 1.091 | 1.714 | 2.128 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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