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产品概况
| 货号 | EK0664 |
|---|---|
| 产品名称 | Mouse BAFF ELISA Kit |
| 产品描述 | For quantitative detection of mouse BAFF in , cell lysates |
| 免疫原 | NSO, A127-L309 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 46.9pg/ml-3000pg/ml |
| 基因名称 | TNFSF13B |
| 基因全名 | tumor necrosis factor (ligand) superfamily, member 13b |
| Uniprot ID | Q9WU72 |
| 指标别名 | B lymphocyte stimulator|B-cell-activating factor|BAFF|BLyS|CD257|Dendritic cell-derived TNF-like molecule|soluble form|TALL-1|TN13B_HUMAN|TNF- and APOL-related leukocyte expressed ligand 1|TNFSF13B|Tumor necrosis factor ligand superfamily member 13b |
| 背景资料 | B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0664-CAP | 96-well plate precoated with anti- mouse BAFF antibody | 1 |
| EK0664-ST | Lyophilized recombinant mouse BAFF standard | 10ng/tubex2 |
| EK0664-DA | Biotinylated anti- mouse BAFF antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 82 | 293 | 1221 | 76 | 287 | 1209 |
| Standard deviation | 6.47 | 15.52 | 67.15 | 7.14 | 16.07 | 72.54 |
| CV(%) | 7.9% | 5.3% | 5.5% | 9.4% | 5.6% | 6% |
Mouse BAFF ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 46.9 | 94 | 188 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.078 | 0.147 | 0.201 | 0.315 | 0.507 | 0.891 | 1.516 | 2.346 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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