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Product Info Summary
| Species | Human |
| Sample Type | Serum, EDTA/Heparin Plasma |
| Volume Required per Well | Min 25 µl |
| Assay Length | 2.25 hours |
| Specificity | All analytes < 1% intra-panel cross-reactivity |
| Assay Type | Planar Based Multiplexed ELISA |
| Detection Method | Chemiluminescent |
| Multiplex Format | 96-well solid plate |
| SKU | MEK2004 |
| Analytes | IL-1α, IL-4, IL-6, TNFα |
Product Overview
The Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-1α, IL-4, IL-6, TNFα). Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
Assay Ranges
| Units | Calibrator Range | LLD Limit | |
| IL-1α | pg/ml | 4000 - 7.1 | 3.55 |
| IL-4 | pg/ml | 1676 - 3 | 1.5 |
| IL-6 | pg/ml | 3000 - 5.3 | 2.65 |
| TNFα | pg/ml | 6500 - 11.5 | 5.75 |
Kit Components
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
Calibrator
Detection Mix
Substrate
Sample Diluents
Streptavidin HRP
Wash Buffer
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