Rat ALCAM ELISA Kit

货号:EK1572
反应性:Rat
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产品概况

货号 EK1572
产品名称 Rat ALCAM ELISA Kit
产品描述 For quantitative detection of rat ALCAM in , cell lysates
免疫原 NSO, W28-K527
交叉反应 There is no detectable cross-reactivity with other relevant proteins.
敏感性 <10pg/ml
检测范围 78pg/ml-5000pg/ml
基因名称 ALCAM
基因全名 activated leukocyte cell adhesion molecule
Uniprot ID O35112
指标别名 ALCAM protein | CD166 antigen | Protein MEMD | Q13740
背景资料 CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the Chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
显色时间 25-30分钟

 

试剂盒内容

SKU 名称 数量
Catalog number Description Quantity
EK1572-CAP 96-well plate precoated with anti- rat ALCAM antibody 1
EK1572-ST Lyophilized recombinant rat ALCAM standard 10ng/tube×2
EK1572-DA Biotinylated anti- rat ALCAM antibody 100μl(dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 100μl(dilution 1:100)
AR1106-1 Sample diluent buffer 30ml
AR1106-2 Antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

 

需要用户自己提供的器材和试剂

  • 1.96孔读板仪器。
  • 2.自动洗板机.
  • 3.移液器和枪头。建议使用多通道移液器/排枪。
  • 4.Eppendorf 管。
  • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

 

批内差/批间差

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 123 659 2375 124 634 2509
Standard deviation 6.39 50.74 187.62 7.93 54.52 225.81
CV(%) 5.2% 7.7% 7.9% 6.4% 8.6% 9%

Rat ALCAM ELISA Kit的典型数据示例(取自最近的一批次质检数据)

(TMB 孵育条件: 37°C, 25-30分钟)

Concentration(pg/ml) 0 78 156 312 625 1250 2500 5000
O.D. 0.004 0.064 0.118 0.215 0.450 0.798 1.578 2.317

*典型数据仅供参考。用户需要自己建立标准曲线。

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