Rat CNTF ELISA Kit

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货号：EK0324

反应性：Rat

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0324-96T	96T	EA	¥2280.00	请登录	请登录

产品概况

货号	EK0324
产品名称	Rat CNTF ELISA Kit
产品描述	For quantitative detection of rat CNTF in , serum and plasma(heparin, EDTA, citrate).
免疫原	E.coli, A2-M200
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	31.2pg/ml-2000pg/ml
基因名称	CNTF
基因全名	ciliary neurotrophic factor
Uniprot ID	P20294
指标别名	AI429687\|Ciliary Neurotrophic Factor\|HCNTF\|MGC41235\|OTTHUMP00000174731\|OTTMUSP00000029848
背景资料	Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
显色时间	25-30分钟

SKU	名称	数量
Catalog number	Description	Quantity
EK0324-CAP	96-well plate precoated with anti- rat CNTF antibody	1
EK0324-ST	Lyophilized recombinant rat CNTF standard	10ng/tube×2
EK0324-DA	Biotinylated anti- rat CNTF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

(TMB 孵育条件： 37°C， 25-30分钟)

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.024	0.049	0.076	0.123	0.268	0.563	1.338	2.294

*典型数据仅供参考。用户需要自己建立标准曲线。

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