货号 | EK0324 |
---|---|
产品名称 | Rat CNTF ELISA Kit |
产品描述 | For quantitative detection of rat CNTF in , serum and plasma(heparin, EDTA, citrate). |
免疫原 | E.coli, A2-M200 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | CNTF |
基因全名 | ciliary neurotrophic factor |
Uniprot ID | P20294 |
指标别名 | AI429687|Ciliary Neurotrophic Factor|HCNTF|MGC41235|OTTHUMP00000174731|OTTMUSP00000029848 |
背景资料 | Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0324-CAP | 96-well plate precoated with anti- rat CNTF antibody | 1 |
EK0324-ST | Lyophilized recombinant rat CNTF standard | 10ng/tube×2 |
EK0324-DA | Biotinylated anti- rat CNTF antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 82 | 276 | 868 | 80 | 293 | 809 |
Standard deviation | 4.59 | 16.83 | 60.76 | 5.52 | 23.14 | 60.67 |
CV(%) | 5.6% | 6.1% | 7% | 6.9% | 7.9% | 7.5% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.024 | 0.049 | 0.076 | 0.123 | 0.268 | 0.563 | 1.338 | 2.294 |
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