Mouse CCL17/TARC ELISA Kit

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货号：EK0685

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0685-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0685
产品名称	Mouse CCL17 ELISA Kit
产品描述	For quantitative detection of mouse CCL17 in , cell lysates
免疫原	E.coli, A24-P93
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	31.2pg/ml-2000pg/ml
基因名称	CCL17
基因全名	chemokine (C-C motif) ligand 17
Uniprot ID	F6R5P4
指标别名	A 152E5.3\|ABCD 2\|C C motif chemokine 17\|C-C motif chemokine 17\|CC chemokine TARC\|CCL 17\|CCL17\|CCL17_HUMAN\|Chemokine (C C motif) ligand 17\|Chemokine ligand 17\|MGC138271\|MGC138273\|SCYA17\|Small inducible cytokine A17\|Small inducible cytokine subfamily A (Cys Cys) member 17\|Small inducible cytokine subfamily A member 17\|Small-inducible cytokine A17\|T cell directed CC chemokine\|Thymus and activation regulated chemokine\|Thymus and activation-regulated chemokine
背景资料	Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.
显色时间	20-25分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0685-CAP	96-well plate precoated with anti- mouse CCL17 antibody	1
EK0685-ST	Lyophilized recombinant mouse CCL17 standard	10ng/tubex2
EK0685-DA	Biotinylated anti- mouse CCL17 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CCL17 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 20-25分钟)

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.040	0.128	0.226	0.441	0.815	1.283	1.836	2.203

*典型数据仅供参考。用户需要自己建立标准曲线。

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