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分子生物学
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细胞生物学
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蛋白质组学
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蛋白凝胶纯化
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生化试剂
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仪器设备
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通用实验耗材
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色谱耗材
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产品概况
| 货号 | BM1621 |
|---|---|
| 产品名称 | Anti-GCG Antibody |
| 基因名 | GCG |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal (Clone: K79bB10) |
| 抗体亚型 | Mouse IgG1 |
| 免疫原 | Polymerized porcine glucagon. |
| 内容 | 200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA |
| 纯化方式 | Ascites |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Glucagon is a member of a multigene family that includes secretin. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. The human glucagon gene is approximately 9.4 kb long and contains 6 exons and 5 introns, and assigned to 2q36-2q37. |
| 研究类别 | 1. Schroeder, W. T.; Lopez, L. C.; Harper, M. E.; Saunders, G. F. : Localization of the human glucagon gene (GCG) to chromosome segment 2q36-37. Cytogenet. Cell Genet. 38: 76-79, 1984. 2. White, J. W.; Saunders, G. F. : Structure of the human glucagon gene. Nucleic Acids Res. 14: 4719-4730, 1986. |
| Uniprot ID | GCG: Human(P01275), Mouse(P55095), Rat(P06883) |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Immunofluorescence: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大 [list_product_images]IHC(P): Rat Pancreas Tissue|Figure 2.IF analysis using anti-GCG antibody (BM1621). detected in paraffin-embedded section of mouse pancreas tissue. The tissue section were stained using the DyLight 594 Conjugated AffiniPure Goat Anti-mouse IgG Secondary Antibody (red)Catalog?#?BA1141) and counterstained with DAPI (blue).[/list_product_images]
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