Mouse CD14 ELISA Kit

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货号：EK0695

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0695-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0695
产品名称	Mouse CD14 ELISA Kit
产品描述	For quantitative detection of mouse CD14 in , cell lysates
免疫原	NSO, A18-P345
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	0.87ng/ml-50ng/ml
基因名称	CD14
基因全名	CD14 molecule
Uniprot ID	P10810
指标别名	CD 14\|CD14 antigen\|CD14 molecule\|Monocyte differentiation antigen CD14\|Myeloid cell-specific leucine-rich glycoprotein
背景资料	CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
显色时间	20-25分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0695-CAP	96-well plate precoated with anti- mouse CD14 antibody	1
EK0695-ST	Lyophilized recombinant mouse CD14 standard	50ng/tubex2
EK0695-DA	Biotinylated anti- mouse CD14 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CD14 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 20-25分钟)

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.069	0.135	0.180	0.274	0.475	0.858	1.283	2.118

*典型数据仅供参考。用户需要自己建立标准曲线。

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