Mouse Cardiotrophin-1 ELISA Kit

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货号：EK0564

反应性：Mouse

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK0564-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK0564
产品名称	Mouse CT-1 ELISA Kit
产品描述	For Quantitative Detection of mouse CT-1 in , serum and plasma(heparin, EDTA).
免疫原	E.coli, S2-A203
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	156pg/ml-10000pg/ml
基因名称	Ctf1
基因全名	cardiotrophin 1
Uniprot ID	Q60753
指标别名	Cardiotrophin-1\|CT-1\|Ctf1
背景资料	Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.
显色时间	20-25分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0564-CAP	96-well plate precoated with anti- mouse CT-1 antibody	1
EK0564-ST	Lyophilized recombinant mouse CT-1 standard	10ng/tube×2
EK0564-DA	Biotinylated anti- mouse CT-1 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Mouse CT-1 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 20-25分钟)

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.006	0.117	0.212	0.381	0.637	1.064	1.476	1.917

*典型数据仅供参考。用户需要自己建立标准曲线。

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