产品概况
货号 | EK0564 |
---|---|
产品名称 | Mouse CT-1 ELISA Kit |
产品描述 | For Quantitative Detection of mouse CT-1 in , serum and plasma(heparin, EDTA). |
免疫原 | E.coli, S2-A203 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | Ctf1 |
基因全名 | cardiotrophin 1 |
Uniprot ID | Q60753 |
指标别名 | Cardiotrophin-1|CT-1|Ctf1 |
背景资料 | Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0564-CAP | 96-well plate precoated with anti- mouse CT-1 antibody | 1 |
EK0564-ST | Lyophilized recombinant mouse CT-1 standard | 10ng/tube×2 |
EK0564-DA | Biotinylated anti- mouse CT-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 270 | 1852 | 6181 | 243 | 1959 | 6792 |
Standard deviation | 17.55 | 79.63 | 438.85 | 16.03 | 84.23 | 584.11 |
CV(%) | 6.5% | 4.3% | 7.1% | 6.6% | 4.3% | 8.6% |
Mouse CT-1 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.006 | 0.117 | 0.212 | 0.381 | 0.637 | 1.064 | 1.476 | 1.917 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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