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产品概况
| 货号 | EK1434 |
|---|---|
| 产品名称 | Rat CDH1/E-Cadherin ELISA Kit |
| 产品描述 | For quantitative detection of rat E-Cadherin in , serum and plasma(heparin). |
| 免疫原 | NSO, Q24-S704 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <15pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | CDH1 |
| 基因全名 | cadherin 1, type 1, E-cadherin (epithelial) |
| Uniprot ID | Q9R0T4 |
| 指标别名 | Arc 1|Cadherin 1|cadherin 1 type 1 E-cadherin|Cadherin1|CAM 120/80|CD 234|CD324|CD324 antigen|CDH1|CDHE|ECAD|Epithelial cadherin| epithelial calcium dependant adhesion protein|LCAM|Liver cell adhesion molecule|UVO|Uvomorulin |
| 背景资料 | Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1434-CAP | 96-well plate precoated with anti- rat E-Cadherin antibody | 1 |
| EK1434-ST | Lyophilized rat E-Cadherin standard | 10ng/tube×2 |
| EK1434-DA | Biotinylated anti- rat E-Cadherin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 278 | 1708 | 5563 | 288 | 1866 | 5045 |
| Standard deviation | 21.4 | 122.97 | 378.28 | 27.36 | 169.8 | 363.24 |
| CV(%) | 7.7% | 7.2% | 6.8% | 9.5% | 9.1% | 7.2% |
Rat CDH1/E-Cadherin ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.097 | 0.100 | 0.163 | 0.252 | 0.482 | 0.930 | 1.654 | 2.438 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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