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Component list is the same as WC Medium with the following exceptions:
• The addition of 1 mL of DYV Metal Solution
• Increase in the volume of Na2SiO3·9H2O stock added to 2 mL
Directions
For 1 L Total
1.To approximately 900 mL of dd-H2O, add the following components in the order listed (not including the vitamins) while stirring continuously.
2.Adjust the pH to 7.8
3.Transfer the contents of the beaker to a 1-Liter graduated cylinder and bring the total volume to 1.0 Liter with dd-H2O.
4.Add the sterile vitamin components and mix well.
5.Do not autoclave medium! Filter sterilize only. 6. Store at refrigerator temperature.
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