产品概况
货号 | EK1123 |
---|---|
产品名称 | Human CCL14 ELISA Kit |
产品描述 | For quantitative detection of human CCL14 in , cell lysates |
免疫原 | E.coli, T20-N93 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | CCL14 |
基因全名 | chemokine (C-C motif) ligand 14 |
Uniprot ID | Q16627 |
指标别名 | C-C motif chemokine 14|CC-1|CC-3|Chemokine (C-C motif) ligand 14|Chemokine CC-1/CC-3|Chemokine CC-3|CKB1|HCC-1|HCC-1(1-74)|HCC-1/HCC-3|HCC-3|MCIF|NCC-2|NCC2|New CC chemokine 2|SCYA14|SCYL2|Small inducible cytokine subfamily A (Cys-Cys), member 14|Small-inducible cytokine A14|SY14 |
背景资料 | Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1123-CAP | 96-well plate precoated with anti- human CCL14 antibody | 1 |
EK1123-ST | Lyophilized recombinant human CCL14 standard | 10ng/tube×2 |
EK1123-DA | Biotinylated anti- human CCL14 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 37 | 238 | 445 | 40 | 227 | 446 |
Standard deviation | 1.92 | 13.8 | 25.81 | 2.08 | 15.89 | 33.45 |
CV(%) | 5.2% | 5.8% | 5.8% | 5.2% | 7% | 7.5% |
Human CCL14 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.007 | 0.069 | 0.161 | 0.359 | 0.670 | 1.288 | 1.955 | 2.234 |
*典型数据仅供参考。用户需要自己建立标准曲线。
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