Human CCL14/HCC-1 ELISA Kit

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货号：EK1123

反应性：Human

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SKU	包装规格	单位	目录价	您的价格	库存	数量	购物车
EK1123-96T	96T	EA	¥2480.00	请登录	请登录

产品概况

货号	EK1123
产品名称	Human CCL14 ELISA Kit
产品描述	For quantitative detection of human CCL14 in , cell lysates
免疫原	E.coli, T20-N93
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<10pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	CCL14
基因全名	chemokine (C-C motif) ligand 14
Uniprot ID	Q16627
指标别名	C-C motif chemokine 14\|CC-1\|CC-3\|Chemokine (C-C motif) ligand 14\|Chemokine CC-1/CC-3\|Chemokine CC-3\|CKB1\|HCC-1\|HCC-1(1-74)\|HCC-1/HCC-3\|HCC-3\|MCIF\|NCC-2\|NCC2\|New CC chemokine 2\|SCYA14\|SCYL2\|Small inducible cytokine subfamily A (Cys-Cys), member 14\|Small-inducible cytokine A14\|SY14
背景资料	Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
显色时间	25-30分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1123-CAP	96-well plate precoated with anti- human CCL14 antibody	1
EK1123-ST	Lyophilized recombinant human CCL14 standard	10ng/tube×2
EK1123-DA	Biotinylated anti- human CCL14 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

Human CCL14 ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 25-30分钟)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.007	0.069	0.161	0.359	0.670	1.288	1.955	2.234

*典型数据仅供参考。用户需要自己建立标准曲线。

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