产品概况
货号 | EK0660 |
---|---|
产品名称 | Mouse AXL ELISA Kit |
产品描述 | For quantitative detection of mouse AXL in , cell lysates |
免疫原 | NSO, H20-P443 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | AXL |
基因全名 | AXL receptor tyrosine kinase |
Uniprot ID | Q00993 |
指标别名 | Adhesion related kinase|AI323647|Ark|AXL oncogene|AXL receptor tyrosine kinase|AXL transforming gene|AXL transforming sequence/gene|JTK11|Oncogene AXL|Tyro7|Tyrosine protein kinase receptor UFO|UFO |
背景资料 | Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants. |
显色时间 | 20-25分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0660-CAP | 96-well plate precoated with anti- mouse AXL antibody | 1 |
EK0660-ST | Lyophilized recombinant mouse AXL standard | 10ng/tubex2 |
EK0660-DA | Biotinylated anti- mouse AXL antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
*小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。
需要用户自己提供的器材和试剂
*样品准备的相关试剂也不含在试剂盒中。
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 53 | 280 | 992 | 48 | 266 | 1046 |
Standard deviation | 3.86 | 14.84 | 66.46 | 3.55 | 14.09 | 81.58 |
CV(%) | 7.3% | 5.3% | 6.7% | 7.4% | 5.3% | 7.8% |
Mouse AXL ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 20-25分钟)
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.063 | 0.090 | 0.135 | 0.201 | 0.345 | 0.655 | 1.155 | 2.066 |
*典型数据仅供参考。用户需要自己建立标准曲线。
Copyright © 2021 新疆宝信源柏生物技术有限公司 版权所有!
新ICP备2022000265号-1 新公网安备 65010502000866号